Detailed view of 4512-JFI-0508

Strain

ID 16
Name LEW/SsNHsd
Type Inbred
Living False
Source Envigo
Note

Animal

Animal_name LEW/SsNHsd_Rat1
Sex M
Age adolescent
Generation
Euthanasia_date 2018-02-01
Source Envigo
Note UTHSC order 6489, foundation stock

Tissue

ID 4512-JFI-0501
Type spleen
Transfer_History Chen to HudsonAlpha
Note None

Sequencing

DNA_source 4512-JFI-0501
Platform 10X Chromium
DNA_extraction_method HMW
DNA_concentration 47
DNA_target_length
DNA_extraction_facility HudsonAlpha
Library_kit 150bp_PE
Library_facility HudsonAlpha
Sequencing_instrument HiSeq_X10
Sequencing_date 2018-07-18
Sequencing_facility HudsonAlpha
Sequencing_notes Lanes per sample: 1
Raw_data_dir_name 4512-JFI-0508
Raw_data_format fastq.gz
Raw_data_file_size_GB 78
Raw_data_available_from Chen
Note None

Analysis

Analysis_ID 4512-JFI-0508.LR1 4512-JFI-0508.DV1
Software_name LongRanger DeepVariant
Software_version 2.2.2 0.9.0-gpu
Results_dir longranger_results/0508_lewssnhsd /data/10_TB/deepvar
Analysis_date 2018-08-08 2019-12-04
Note

LongRanger results

Analysis_ID 4512-JFI-0508.LR1
instrument_ids E00438
gems_detected 1570629
mean_dna_per_gem 485313.0739
bc_on_whitelist 0.8972867992
bc_mean_qscore 38.38699649
n50_linked_reads_per_molecule 12
corrected_loaded_mass_ng 1.141638525
snps_phased 0.8798655896
genes_phased_lt_100kb 0.735
longest_phase_block 553440
n50_phase_block 51839
molecule_length_mean 24942.34891
molecule_length_stddev 41126.5233
number_reads 863463482
median_insert_size 336
mean_depth 37.73594046
zero_coverage 0.02518567755
mapped_reads 0.9282834106
pcr_duplication 0.05548531515
on_target_bases
r1_q20_bases_fract 0.9382990324
r1_q30_bases_fract 0.8780083054
r2_q20_bases_fract 0.8460678633
r2_q30_bases_fract 0.7323306717
si_q20_bases_fract 0.9652593354
si_q30_bases_fract 0.9403095975
bc_q20_bases_fract 0.9926969278
bc_q30_bases_fract 0.9793696081
large_sv_calls 2361
short_deletion_calls 32220

Deepvariant results

index 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220
Analysis_ID 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1 4512-JFI-0508.DV1
Chr chr10 chr11 chr12 chr13 chr14 chr15 chr16 chr17 chr18 chr19 chr1 chr20 chr2 chr3 chr4 chr5 chr6 chr7 chr8 chr9 chrX chrY
Biallelic_Deletion 51063 29181 33625 38165 50543 40453 32813 45378 30923 27583 97321 29972 114944 76367 72523 82904 63043 59583 53743 46162 43465 156
Biallelic_Insertion 51638 30786 31049 39469 49229 40904 32748 43185 31717 26934 99464 28887 112372 75228 71927 80263 62766 58822 53609 46337 42578 306
Biallelic_SNP 182417 102150 124144 91737 226551 150124 97061 187469 93200 92769 393653 118274 569189 314603 297359 347805 266627 255726 197917 167115 106589 782
Multiallelic_Complex 1547 789 875 1004 1347 1055 857 1147 835 822 2857 753 2874 2026 1871 1974 1594 1731 1458 1286 591 9
Multiallelic_Deletion 3455 1297 1722 1416 2487 1572 1186 2428 1328 1298 4596 1211 6022 4102 3882 4264 3046 3307 2823 2054 791 9
Multiallelic_Insertion 5881 2193 3042 2150 4311 2746 1975 4173 2222 2145 7808 2122 10298 7141 6425 7415 5599 5666 4871 3500 1301 8
Multiallelic_SNP 109 59 156 35 118 128 73 146 47 230 386 138 485 194 129 144 315 203 130 199 87 1
RefCall 339593 223638 216079 277012 322653 289859 230238 291997 215540 206314 798537 193387 785946 508139 478671 498802 450260 426155 365968 338564 344324 23486
Transition 118447 62783 79370 52465 140632 89391 58476 120510 57751 56806 237699 74386 348574 196666 184346 216427 160911 160091 126412 100670 56491 450
Transversion 63970 39367 44774 39272 85919 60733 38585 66959 35449 35963 155954 43888 220615 117937 113013 131378 105716 95635 71505 66445 50098 332

Supernova results